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Image Search Results
Journal: Frontiers in Microbiology
Article Title: Dissection of the cis -2-decenoic acid signaling network in Pseudomonas aeruginosa using microarray technique
doi: 10.3389/fmicb.2015.00383
Figure Lengend Snippet: Induction of planktonic mode of growth in P. aeruginosa PAO1 biofilms by CDA. (A) Determination of cell density using semi-batch cultures. P. aeruginosa biofilms were grown for 5 days in petri dishes. Dispersion by CDA was tested at concentrations between 100 and 620 nM (carrier used as a control). Cell density was determined by measuring the optical density. (B) Determination of cell density using continuous cultures. Biofilms of P. aeruginosa PAO1 were grown in flow cell continuous cultures, and dispersed by given CDA concentrations or the carrier. Effluent runoffs were then collected and cell density was determined. (C) Quantification of percent surface coverage. P. aeruginosa PAO1 biofilms remaining on the surface were then stained with 3 μl/ml SYTO 9 to allow analysis using fluorescence microscopy and percent surface coverage was quantified using digital image analysis. Error bars indicate SD ( n = 3). (D) Stained biofilms before and after treatments by CDA. Images are top-down views (x-y plane); scale bars: 50 μm.
Article Snippet:
Techniques: Dispersion, Control, Staining, Fluorescence, Microscopy
Journal: Frontiers in Microbiology
Article Title: Dissection of the cis -2-decenoic acid signaling network in Pseudomonas aeruginosa using microarray technique
doi: 10.3389/fmicb.2015.00383
Figure Lengend Snippet: Functional classifications of DE genes in P. aeruginosa after treatment with 100 nM CDA. (A) DE genes were classified in to more than 15 functional groups. The top functional classes with the percentages of genes altered in each class were presented in the pie graph. (B) Percentages of up and down regulated genes in each functional group.
Article Snippet:
Techniques: Functional Assay
Journal: Frontiers in Microbiology
Article Title: Dissection of the cis -2-decenoic acid signaling network in Pseudomonas aeruginosa using microarray technique
doi: 10.3389/fmicb.2015.00383
Figure Lengend Snippet: Comparison of microarray and semi-qRT PCR analyses of 5 selected genes in P. aeruginosa biofilm cells exposed to 100 nM CDA and in untreated biofilm cells . Error bars indicate SD ( n = 3).
Article Snippet:
Techniques: Comparison, Microarray, Quantitative RT-PCR
Journal: Frontiers in Microbiology
Article Title: Dissection of the cis -2-decenoic acid signaling network in Pseudomonas aeruginosa using microarray technique
doi: 10.3389/fmicb.2015.00383
Figure Lengend Snippet: Removal of established biofilms of P. aeruginosa , using CDA combined by antimicrobial treatments . Following dispersion of biofilms by CDA, cells remaining on the surface were easily killed and removed by various antimicrobial compounds (tobramycin; Tob, H 2 O 2 , ciprofloxacin; Cip). (A) LIVE/DEAD staining of biofilms. 48 h-old-biofilms of P. aeruginosa were treated with indicated antimicrobials alone or combined with 100 nM CDA for only 1 h. Biofilms were then stained with LIVE/DEAD staining and (B) Quantification of percent surface coverage. After LIVE/DEAD staining percent surface coverage was quantified using digital image analysis. Images are top-down views (x-y plane); scale bars: 50 μm. Error bars indicate SD ( n = 3).
Article Snippet:
Techniques: Dispersion, Staining
Journal: Frontiers in Microbiology
Article Title: Dissection of the cis -2-decenoic acid signaling network in Pseudomonas aeruginosa using microarray technique
doi: 10.3389/fmicb.2015.00383
Figure Lengend Snippet: Comparison of predicted and known proteins involved in CDA and DSF synthesis and perception. (A) CDA synthesis and perception in P. aeruginosa based on known PPIs. (B) Demonstrated DSF synthesis and perception in Xcc .
Article Snippet:
Techniques: Comparison